44 by: Ella Johnson

Student – Ella Johnson

Enzyme: Lipase

E.C. Number: 3.1.1.3

Lipase can be found in the mouth, stomach, and pancreas however, for this video, I will specifically be talking about lipase of the stomach, gastric lipase. Gastric lipase is secreted from
the stomach’s mucosa by gastric chief cells. Other forms of lipase in the pancreas and mouth have differences in regards to their reaction mechanisms. Lipase works to break down consumed triglycerides into their components, 3 fatty acid chains and one glycerol backbone. The fatty acids and glycerol are bound together by ester bonds, therefore lipase works by a hydrolysis mechanism to break these ester bonds. By breaking down triglycerides, lipase is making it easier for the molecule to be more easily broken down and absorbed by the body. Water and the catalytic triad of the enzyme are critical for the hydrolysis of triglycerides. Without lipase, fats would accumulate in the bloodstream and eventually become problematic for the normal function of the human body.

References:

Casas-Godoy L, Castillo E, Sandoval G. 2015. Medium-engineering: a useful tool for modulating lipase activity and selectivity. [cited 2024 Oct 9]. Available from: https://ciatej.repositorioinstitucional.mx/jspui/bitstream/1023/694/1/Medium-engineering.pdf doi: 10.1515/boca-2015-0013
Berchansky A, Canner D, Clark K, Harel M, Hodis E, Martz E, Murray Q, Schell S, Sussman J, Tadesse L, Ziegler N. 2023. Lipase. Proteopedia. [cited 2024 Oct 9]. Available from:
https://proteopedia.org/wiki/index.php/Lipase#:~:text=Lipase%20Catalytic%20Mechanism,-Lipase%20activation%20at&text=This%20initiates%20active%20site%20binding,resi
dues%20like%20the%20catalytic%20triad
Bordes F, Casas-Godoy L, Duquesne S, Gasteazoro F, Marty A, Sandoval G. 2018. Lipases: An Overview. ResearchGate. [cited 2024 Oct 9]. Available from: https://www.researchgate.net/publication/334248536_Lipases_An_Overview