21 by: Aurora Lewis

Student – Aurora Lewis

Enzyme & EC # – Carboxylate Reductase; E.C: 1.2.1.30

Carboxylate reductase can be found in bacteria and fungi and are typically used for many metabolic processes, which can be related to energy production and adaptation to nutrient availability.

Carboxylate Reductase catalyzes the reduction (ATP and NADPH dependent) of carboxylic acid to aldehydes. This includes three key steps, adenylation of bound carboxylic acid substrate to form AMP intermediate, formation of thioester linkage between phosphopantetheine group and the reduction of the thioester intermediate to the aldehyde.

There are many versions of Carboxylate Reductase, in my story I include the phosphopantetheine group however not every Carboxylate Reductase Enzyme uses it.

References

Finnigan, W., Thomas, A., Cromar, H.. Gough, B,. Snajdrova, R., Adams, J., Littlechild, J.,Harmer,N,. (2016). Characterization of carboxylic acid reductases as enzymes in the toolbox for synthetic chemistry. ChemCatChem, 9(6), 1005–1017.
https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cctc.201601249
Gahloth, D., Dunstant, M., Quaglia, D., Klumbys, E., Lockhart-Cairns, M., Hill, A., Derrington, S., Scrutton, N., Turner, N., Leys. D., (2017). Structures of carboxylic acid reductase reveal domain dynamics underlying catalysis. Journal of Biological Chemistry, 295(4), 1234-1245.
https://pmc.ncbi.nlm.nih.gov/articles/PMC5563451/